Figure 5.

TGF-β can regulate Tbet expression in liver resident and blood NK cells. Liver conditioned media (LCM) was generated from healthy donor liver biopsies. ELISA was performed for the cytokines TGF-β and IL-10. (A) Concentration of cytokines TGF-β and IL-10 from matched LCM samples (n = 10). CD56^bright Eomes^hi Tbet^lo NK cells FACS isolated from liver perfusate (LP) were cultured for 7 days, supplemented with 10% v/v liver conditioned media (LCM), IL-10 (10 ng/ml) or TGF-β (5 ng/ml) and acquired on a FACS Fortessa. (B) Representative histogram of Tbet expression from cells at day 7 untreated (blue line), 10% LCM (orange line), IL-10 (red line), and TGF-β (green line). (C) MFI of Tbet in CD56^bright NK cells from LP at day 7 with LCM, IL-10 or TGF-β treatments. (D) MFI of Eomes in CD56^bright NK cells from LP at day 7 with LCM, IL-10 or TGF-β treatments. (E) Representative histogram of Tbet expression from blood NK cells at day 7 untreated (blue line) or TGF-β (green line). (F) MFI of Tbet in PB CD56^bright NK cells at day 7 with or without TGF-β. (G) MFI of Eomes in PB CD56^bright NK cells at day 7 with or without TGF-β. Data presented as mean ± SEM (A) or box and whisker plots with minimum and maximum values (C,D,F,G). Data was analyzed using Friedman test, with Dunn's multiple comparison test or paired t-test (n = 5; *p < 0.05 and **p < 0.01).