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. 2019 Jul 3;10:1518. doi: 10.3389/fimmu.2019.01518

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Copyright © 2019 Adankwah, Lundtoft, Güler, Franken, Ottenhoff, Mayatepek, Owusu-Dabo, Phillips, Nausch and Jacobsen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Two-hit assay induced Mtb latency antigen specific IFN-γ positive CD4⁺ T cells discriminate TB patients and HCs. Receiver Operator Characteristic (ROC) analysis for classification of TB patients and HCs using Rv1733 and Rv2628 specific IFN-γ positive CD4⁺ T cells of TB patients and HCs after two-hit stimulation. Area Under Curve (AUC) values of ROC analysis for the study groups (A) and an independent test cohort (B) [TB patients (n = 4) and HCs (n = 13)]. Deduced AUC-values and nominal p-values are shown as **p < 0.01, ***p < 0.001, and ****p < 0.0001. ns: not significant.