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. 2019 Jul 5;132(13):1572–1581. doi: 10.1097/CM9.0000000000000296

Figure 5.

Figure 5

RFX5-YWHAQ inhibits apoptosis by regulating p53 and downstream pathway in HCC. (A and B) HepG2 (A) and SK-HEP-1 cells (B) transduced lentiviral vector control, RFX5 or YWHAQ were treated with 5 nmol/L ActD and subjected to apoptosis analysis by one-way ANOVA. Data were shown as mean ± SD (n = 3). (C and D) HepG2 (C) and SK-HEP-1 cells (D) transduced lentiviral sgRNA or YWHAQ were treated with 5 nmol/L ActD and subjected to apoptosis analysis by one-way analysis of variance. Data were shown as mean ± SD (n = 3). (E and F) Total proteins from HepG2 (E) and SK-HEP-1 cells (F) transduced lentiviral vector control, RFX5 or YWHAQ were subjected to Western blotting analysis of indicated proteins. (G and H) Total proteins from HepG2 (G) and SK-HEP-1 cells (H) transduced lentiviral sgRNA or YWHAQ were subjected to Western blotting analysis of indicated proteins. ‡P < 0.05, †P < 0.01, ∗P < 0.001. ActD: Dactinomycin D; HCC: Hepatocellular carcinoma; RFX5: Regulatory factor X-5; SD: Standard deviation.