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. 2019 Jul 3;10:1281. doi: 10.3389/fmicb.2019.01281

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Copyright © 2019 Kay, Edwards, Brown and Dixon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Schematic of the imaging setup used to record time-lapse photography of larvae 1: Black felt shroud to reduce the effects of natural light on quality of images; 2: Camera: Logitek C100 5 mp; 3: table lamp for consistent lighting; 4: perspex incubator: Stuart Scientific SI140; 5: Greaseproof paper for better contrast in images. (B) Automated syringe pump setup used for inoculating larva. (C) Injections were administered through the rear pro-leg. (D) Example of image output from SkyStudio Pro time-lapse software.