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Schematic for cell-free production of self-assembled nanolipoprotein particles (NLPs). Constituents (DNA, lipids, and cell-free extracts) are combined in a single reaction vial. The cell-free lysates utilize T7-coupled transcription and translation to produce fully formed disc shaped nanoparticles as imaged with transmission electron microscopy (TEM) (inset). The white scale bars are 20 nm.
