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Schematic of two approaches for cell-free production of membrane proteins solubilized in NLPs: (A) The addition of fully formed NLPs, DNA encoding the membrane protein of interest, and additional lipid to a cell-free extract results in NLP solubilized receptor (Katzen et al., 2008). (B) Co-translation uses the addition of only plasmids encoding the ApoA1 and membrane protein of interest and lipid to a cell-free extract resulting in NLP solubilized receptor (Cappuccio et al., 2008).
