Figure 5.

Effects of PP inhibitors combined with PK inhibitors on the NKA-sAHPs. a, Simultaneous PP-1, PP-2A, and PP-2B inhibition in condition of inhibited PKs. The slices were pretreated with 2 μm KT5823 and 5 μm chelerythrine to inhibit both PKG and PKC. The two overlaid traces represent the control NKA-sAHPs in the same neuron before (Control; black) and after applying together 1 μm OA and 10 μm FK506 (red). Bar represents the normalized results of five experiments (mean ± SEM) for NKA-sAHP amplitudes (green) and areas (blue). b, Same as in a, but in slices pretreated only with the PKC inhibitor chelerythrine (5 μm; n = 6). c, Same as in a, but in slices pretreated only with the PKG inhibitor KT5823 (2 μm; n = 6). Inhibiting all three PPs, which normally suppressed the NKA-sAHPs, had no effect on condition of inhibited PKG and PKC. When only one of these two PKs was inhibited, then subsequent inhibition of all three PPs suppressed the NKA-sAHPs. Thus, both PKG and PKC mediate tonic phosphorylation of NKAs. **p < 0.01, ***p < 0.001, ns, not significant.