Suprathreshold Activation of Delayed Firing GRPRexcit Neurons during Repetitive Burst-like Stimulation of GRP Neurons Depends on GRP Release
(A) Experimental setup. Grp-ChR2 neurons were excited with repetitive burst-like blue light stimuli and targeted current-clamp recordings were made from delayed firing GRPRexcit neurons.
(B) Left: representative voltage traces in GRPRexcit neurons during 5 min burst-like light stimulation of GRP neurons (blue line) in the presence of NBQX (20 μM) and AP-5 (50 μM). Inset: incidence of GRPRexcit neuron firing during burst-like light stimulation (n = 10 cells from 7 mice). Right: paired plot showing RMP before (black) and 5 min after (blue) repetitive burst-like light stimulation. Circles are individual cells (n = 10 from 7 mice). Two-tailed paired t test, p = 0.001.
(C) Left: same as (B) but in the presence of the GRPR blocker DPDMB (1 μM). Paired two-tailed t test, p = 0.021.
(D) Same (B) but in the combined presence of NBQX, AP-5, and DPDMB. Two-tailed paired t test, p = 0.39.
All error bars indicate SEM.