Table 1.
Passive and Active Biophysical Properties of Grp-eGFP and Grpr-eGFP Neurons
| RMP (mV) | Cm (pF) | Rinput (MΩ) | Rheobase (pA) | Action Potential |
||||
|---|---|---|---|---|---|---|---|---|
| Threshold (mV) | Amplitude (mV) | Width (ms)a | AHP (mV) | |||||
| GRP (n = 31) | −65.9 ± 1.2 | 37.0 ± 2.3 | 1682 ± 104 | 12.6 ± 1.2 | −38.4 ± 0.5 | 69.0 ± 1.7 | 3.71 ± 0.14 | −29.6 ± 0.8 |
| Versus GRPR delayed | ∗∗∗ | — | ∗∗∗ | ∗∗∗ | ∗∗ | — | ∗∗∗ | — |
| Versus GRPR tonic | — | — | ∗ | — | ∗∗∗ | — | ∗∗∗ | — |
| Versus GRPR phasic | ∗∗∗ | — | ∗∗∗ | ∗ | ∗∗∗ | — | ∗∗∗ | — |
| Versus GRPR gap | — | — | — | — | — | — | ∗∗∗ | ∗ |
| Versus GRPR initial bursting | — | — | — | — | — | — | ∗∗ | — |
| GRPR delayed (n = 53) | −73.3 ± 0.7 | 40.9 ± 2.0 | 1064 ± 49 | 28.1 ± 2.1 | −41.5 ± 0.4 | 67.0 ± 2.8 | 2.18 ± 0.05 | −29.1 ± 0.7 |
| Versus GRPR tonic | — | — | ∗∗∗ | ∗∗∗ | — | — | — | — |
| Versus GRPR phasic | — | — | ∗∗∗ | — | ∗∗∗ | — | — | — |
| Versus GRPR gap | — | — | ∗∗∗ | — | — | — | — | ∗ |
| Versus GRPR initial bursting | — | — | ∗∗∗ | — | — | — | — | — |
| GRPR tonic (n = 19) | −70.2 ± 0.9 | 44.8 ± 3.7 | 1197 ± 92 | 8.21 ± 1.59 | −43.8 ± 1.2 | 78.4 ± 1.5 | 2.22 ± 0.10 | −28.5 ± 1.7 |
| Versus GRPR phasic | — | — | ∗∗ | ∗∗ | — | — | — | — |
| Versus GRPR gap | — | — | — | — | — | — | — | ∗ |
| Versus GRPR initial bursting | — | — | — | — | — | — | — | — |
| GRPR phasic (n = 7) | −77.5 ± 2.8 | 35.6 ± 6.5 | 650 ± 160 | 28.3 ± 5.1 | −47.4 ± 1.3 | 75.2 ± 3.2 | 1.83 ± 0.15 | −23.8 ± 3.0 |
| Versus GRPR gap | — | — | — | — | — | — | — | ∗∗∗ |
| Versus GRPR initial bursting | — | — | — | — | ∗∗ | — | ∗ | — |
| GRPR gap (n = 6) | −71.0 ± 2.2 | 48.5 ± 8.7 | 1001 ± 119 | 20.0 ± 3.6 | −41.8 ± 1.0 | 80.6 ± 1.5 | 1.98 ± 0.15 | −37.1 ± 2.0 |
| Versus GRPR initial bursting | — | — | — | — | — | — | — | — |
| GRPR initial bursting (n = 6) | −70.4 ± 1.8 | 43.4 ± 4.2 | 990 ± 127 | 20.17 ± 3.6 | −39.7 ± 1.5 | 62.0 ± 7.5 | 2.85 ± 0.26 | −27.9 ± 3.3 |
RMP, resting membrane potential, Cm, membrane capacitance, Rinput, input resistance, AHP, afterhyperpolarization. Values are means ± SEM. One-way ANOVA followed by a Bonferroni post hoc test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. F(5,116) = 9.24 (RMP); 1.23 (Cm); 162 (Ri); 11.85 (rheobase); 10.6 (AP threshold); 2.622 (action potential amplitude); 40.08 (action potential width); 3.90 (afterhyperpolarization).
a
Determined at the action potential base.