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. 2019 Jul 9;9:9896. doi: 10.1038/s41598-019-45885-7

Figure 1.

Figure 1

Analytical size exclusion of CraCRY-∆CTE (a) and WT (b). In both cases most (80–97%) of the protein sample elute in the monomeric state. (c) UV/Vis measurement of CraCRY WT treated with blue light (BL) and different concentrations of dithiothreitol (DTT) or tris(2-carboxyethyl)-phosphine (TCEP). The FAD cofactor was reduced to FADH− under all conditions (except only BL), but in the sample treated with 12.5 mM DTT less degradation can be observed. (d) Difference spectra of the photoreduction of CraCRY WT with 12.5 mM DTT and 30 min BL as well as reoxidation of FADH− in- and outside the light chamber. After 18 h the sample outside the light chamber entirely reoxidized to FADOX (red line), whereas for the sample inside the chamber 70% remain in the FADH− state with some contributions from FADH° and FADOX (blue line).