Figure 2.

Selected heat maps of the relative fractional deuterium uptake of CraCRY-WT minus CraCRY∆CTE. The relative uptake of the FAD_OX state at 10 s was mapped onto the CraCRY∆CTE structure (PDB: 5ZM0). Red sections correlate with a higher deuterium uptake in full-length CraCRY, while blue sections correlate with a higher uptake in the ∆CTE variant than in WT. For the black segments no peptides could be assigned by HDX-MS analyses. Changes were observed for the linker between the N- and C-terminal domains and for a helix bundle in the C-terminal domain (α10 to α13) that is exposed at the protein surface. This correlates to a higher flexibility or solvent accessibility of these regions in the WT.