Figure 7.

DNA polymerization activity. (a,b) DNA polymerization activity on gapped DNA of PolXBs variants. Assays were performed as described in Materials and Methods, incubating 10 nM of either a 1-nt (a) or a 5-nt (b) gapped DNA with the indicated polymerase concentration. Samples were incubated at 30 °C for either 1 min (a) or 10 min (b). Products were resolved by denaturing PAGE and visualized with a Typhoon 9410 scanner (GE Healthcare) (c,d) DNA polymerization activity of the Polβ-like core of PolXBs on gapped DNA. Assays were performed as described in Materials and Methods, incubating either 4 nM of a 1-nt gapped DNA (c) or 10 nM of a 5-nt gapped DNA (d) with the indicated amount of either the wild-type or the ΔPHP PolXBs variant¹⁹. Samples were incubated at 30 °C for 10 min and analyzed as described above. Full length gels are presented in Fig. S6.