Figure 2.

Antibody Expression from the AAV-Based Expression Plasmid

(A–C) Antibodies were detected using ELISA with various envelope protein derivatives as the immobilized antigen and an HRP-conjugated secondary antibody. Monoclonal antibodies expressed and purified from non-AAV expression plasmids (pControl) were used as positive controls throughout. Error bars: SEM. (A) Antibodies VRC01 and PGT121 were detected using gp120 from a subtype C consensus (ConC) env sequence as the immobilized antigen.⁴⁶ (B) Antibodies CAP228-16H and CAP228-19F were detected using a V1V2 scaffolded protein as the immobilized antigen. (C) CAP256 antibodies were detected using a recombinant gp140 envelope trimer (BG505 SOSIP.664 His6)⁴⁷ as the immobilized antigen captured on nickel-coated plates. (D) A representative western blot for CAP256.16 using a conjugated anti-human Fc antibody for detection. NR, non-reducing; R, reducing conditions.