Table 2.

Significant effects of age and water temperature on small chain fatty acid concentration, NH₃-N concentration, and pH values.

Item	Main Effects Two-Way ANOVA
	Age	Water Temperature	Interaction
Acetic acid	***	NS	NS
Propionic acid	***	NS	NS
Butyric acid	***	NS	*
Isobutyric acid	***	**	NS
Valeric acid	*	NS	NS
Isovaleric acid	*	NS	**
NH3-N	**	NS	NS
pH	NS	NS	NS

*** p < 0.001; ** p < 0.01; * p < 0.05; NS, p > 0.05.