Table 3.

Age-related change of richness and diversity of the cecal microbiota.

Alpha Diversity	Main Effects Two-Way ANOVA
	Age	Water Temperature	Interaction
OTUs	***	NS	NS
ACE	***	NS	NS
Chao	***	NS	NS
Simpson	**	NS	NS
Shannon	***	NS	NS

*** p < 0.001; ** p < 0.01; * p < 0.05; NS, p > 0.05. OTU, operational taxonomic unit.