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Primers used for PCR and qPCR.
| Accession No | Gene | Primers Sequence (5′->3′) | Product Length (bp) | Annealing Temperature (°C) |
|---|---|---|---|---|
| XM_024979243.1 | IZUMO1 (For cloning) |
F: TTCGAGTTGGAAGGACGTGG R: TTCCTTTGAAACCCCCGAGT |
1320 | 59.97 59.15 |
| XM_024979242.1 | IZUMO1 (For gene expression) | F: GCGTTCTAACCGCGATCCTT R: CTCGACTGCCAGAGCTGAAC |
81 | 60.80 60.74 |
|
NM_001034034.2
F: AATGAAAGGGCCATCACCATC |
GAPDH | F: AATGAAAGGGCCATCACCAT R: CACCACCCTGTTGCTGTAGCCA |
204 | 55.85 60.00 |
