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. 2019 Jul 9;9:9936. doi: 10.1038/s41598-019-46408-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reversal of apical-basal polarity using Matrigel beads. (A) Representative image of Matrigel beads in oil (30–60 μm diameter). (B) Brightfield image showing Matrigel bead surrounded by cells in an agarose microwell (10X). (C) Fluorescent images (40X) showing a continuous monolayer of cells surrounding a Matrigel bead shown by the DAPI stain (blue) and reversal of polarity with actin (green) and tight junction protein ZO-1 (red) expressed on the outer membrane away from the Matrigel bead.