Table 2.

Primer sequences used and the name and function of proteins they encode.

Gene	Primer Sequence	Protein encoded	Function/Localization
MDR1	F 5′-GCC AAA GCC AAA ATA TCA GC 3′ R 5′-TTC CAA TGT GTT CGG CAT 3′	P-glycoprotein (P-gp)	Efflux Transporter/Apical membrane
ABCG2	F 5′-TGC AAC ATG TAC TGG CGA AGA 3′ R 5′-TCT TCC ACA AGC CCC AGG 3′	Breast cancer resistance protein (BCRP)	Efflux Transporter/Apical membrane
ABCC2	F 5′-TGA GCA AGT TTG AAA CGC ACAT 3′ R 5′-AGC TCT TCT CCT GCC GTC TCT 3′	Multi-resistance protein 2 (MRP2)	Efflux Transporter/Apical membrane
ABCC3	F 5′-CAC CAA CTC AGT CAA ACG TGC 3′ R 5′-GCA AGA CCA TGA AAG CGA CTC 3′	Multi-resistance protein 2 (MRP3)	Efflux Transporter/Basal membrane
CCND1	F 5′-CAATGACCCCGCACGATTTC 3′ R-CATGGAGGGCGGATTGGAA 3′	Cyclin D1	Cell cycle progression in early G1 phase/cytoplasm
SLC15A	F 5′-TGTCCACCGCCATCTACCATA 3′ R 5′-CCACGAGTCGGCGATAAGAG 3′	Peptide transporter 1 (PEPT 1)	Uptake transporter responsible for the absorption of dietary di- and tripeptides from the small intestinal lumen/apical membrane
GSTA1	F 5′-AGCCGGGCTGACATTCATCT 3′ R 5′-TGGCCTCCATGACTGCGTTA 3′	Glutathione-transferase A 1	Detoxification of electrophilic compounds/ endoplasmic reticulum and outer mitochondrial membrane
APOA1	F 5′-CCAAAAAGCAGCTAGTGAAACC 3′ R 5′-AGTTGCAGTGCGGATGGAA 3′	Apolipoprotein A-I	Phospholipid transporter/apical membrane
TJP1	F 5′-ACC AGT AAG TCG TCC TGA TCC 3′ R 5′-TCG GCC AAA TCT TCT CAC TCC 3′	Zonula Occludin (ZO-1)	Tight Junction scaffolding protein/apical membrane
OCLN	F 5′-CGGG CGA GTC CTG TGA TGA G 3′ R 5′-TCT TGT ATT CCT GTA GGC CAG T 3′	Occludin	Tight Junction Protein/apical membrane
L19	F 5′-TCGCCTCTAGTGTCCTCCG 3′ R 5′-GCGGGCCAAGGTGTTTTTC 3′	Ribosomal protein	Housekeeping