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. 2019 Jul 9;9:9904. doi: 10.1038/s41598-019-46410-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Longitudinal bioluminescence imaging on days 7, 12, 14 and 19. The scale bar indicated standardised radiance across the imaging sessions. The animal shown in Column E did not show significant bioluminescence signal during the longitudinal imaging sessions and no demonstrable tumour by HF-US or post-sacrifice necropsy. Columns B, F and J showed comparatively low signals and corresponding smaller tumours. All other animals had easily demonstrable liver tumours. Animal H was sacrificed on day 16 due to the display of abnormal behaviour.