Fig. 4.

Cellular localization of IL-1β immunoreactivity in the dorsal horn. A–L Cell-type-specific immunolabeling of IL-1β in the ipsilateral dorsal horn at 4 h after intraplantar BmK I. A, E, I IL-1β-positive staining. B, C, J, K Absence of IL-1β co-localization with either the astrocytic marker GFAP or the neuronal marker NeuN. F–G Double immunofluorescence in the superficial dorsal horn showing co-localization of IL-1β with the microglial marker Iba-1. White open squares in C, G, K indicate the magnified images in D, H, L. Scale bars, 50 μm in A–C, E–G, I–K; 20 μm in D, H, L.