Fig. 7.

Effects of BBG on the activation of P2X7Rs and the release of IL-1β in the spinal dorsal horn in the presence of BmK I. Representative P2X7R and IL-1β immunofluorescence labeling at 4 h following sham (A, E) and BmK I stimulation in the saline and BBG injection groups (B–C, F–G). Scale bars, 100 μm. Western blot analysis showed that the BmK I-induced up-regulation of P2X7Rs (D) and IL-1β (H) was inhibited by BBG treatment (100 μmol/L, 30 min before BmK I injection; n = 3/group; *P < 0.05, **P < 0.01, ***P < 0.001 compared with sham, one-way ANOVA, Tukey’s post hoc test).