Table 3.
Effect of dietary supplementation with dehydrated alfalfa or fresh grass on peri-weaning period on relative abundances (%) of the different phyla and on Ruminococcaceae/Lachnospiraceae family ratio and Firmicutes/Bacteroidetes ratio of rabbit caecal microbiota at 30 and 45 days.
Phyla | Experimental Groups 1 | CV 2 | p 3 | ||||||
---|---|---|---|---|---|---|---|---|---|
30 Days | 45 Days | ||||||||
C | A | G | C | A | G | Age | Feed | ||
Firmicutes | 65.78 c | 72.07 ab | 62.38 c | 78.87 b | 83.64 a | 79.98 b | 6.65 | *** | * |
Bacteroidetes | 28.15 a | 24.83 a | 30.41 a | 14.15 b | 12.75 b | 13.26 b | 4.32 | *** | ns |
Proteobacteria | 4.50 a | 1.59 b | 1.92 b | 3.02 ab | 2.30 ab | 3.48 ab | 1.04 | * | * |
Tenericutes | 0.76 b | 1.19 b | 4.22 a | 3.02 a | 1.10 b | 2.28 ab | 0.82 | * | ns |
Actinobacteria | 0.78 ab | 0.29 b | 1.05 a | 0.85 ab | 0.17 b | 0.96 ab | 0.63 | * | ns |
Spirochaetae | 0.03 | 0.03 | 0.02 | 0.09 | 0.04 | 0.04 | 0.05 | ns | ns |
Firmicutes/Bacteroidetes | 2.34 d | 2.90 c | 2.05 d | 5.57 b | 6.56 a | 6.03 ab | 1.02 | ** | * |
Ruminococcaceae/Lachnospiraceae | <0.001 d | 1.58 b | 0.03 d | 0.77 c | 24.32 a | 0.07 d | 3.12 | ** | * |
1 C = rabbit fed pelleted feed only; A = rabbit fed pelleted feed + dehydrated alfalfa; G = rabbit fed pelleted feed + fresh grass. 2 CV = coefficient of variation. 3 ns = not significant. a–d Values within a row with different superscript letters differ significantly as followed reported: *** p < 0.0001, ** p < 0.001, * p < 0.01.