REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Goat anti-human IgG (Fc) AP	Jackson ImmunoResearch Labs	CAT#109-055-008; RRID:AB_2337601
Goat anti-human IgG (Fc) PE	Jackson ImmunoResearch Labs	CAT#109-115-098; RRID:AB_2337675
Goat anti-human IgG (Fc) Alexa Fluor 488	Jackson ImmunoResearch Labs	CAT#109-545-098; RRID:AB_2337840
Rat anti-mouse CD16/32	eBioscience (Thermo Fisher Scientific)	CAT#14-0161-86; RRID:AB_467135
Rat anti-mouse CD4 APC-eFluor®780	eBioscience (Thermo Fisher Scientific)	CAT#47-0042-82; RRID:AB_1272183
Armenian hamster anti-mouse CD11c APC	eBioscience (Thermo Fisher Scientific)	CAT#17-0114-82; RRID:AB_469346
Mouse anti-mouse MHC-II FITC	eBioscience (Thermo Fisher Scientific)	CAT#11-5320-82; RRID:AB_2572501
Rat anti-mouse CD86 PE	eBioscience (Thermo Fisher Scientific)	CAT#12-0862-83; RRID:AB_465769
Rat anti-mouse CD4 FITC	eBioscience (Thermo Fisher Scientific)	CAT#11-0041-85; RRID:AB_464893
Rat anti-mouse CD8a PE	BD Bioscience	CAT#561095; RRID:AB_2034011
Rat anti-mouse CD62L PE-Cy7	eBioscience (Thermo Fisher Scientific)	CAT#25-0621-82; RRID:AB_469633
Armenian hamster anti-mouse CD69 APC	eBioscience (Thermo Fisher Scientific)	CAT#17-0691-82; RRID:AB_1210795
Rat anti-mouse CD8a FITC	eBioscience (Thermo Fisher Scientific)	CAT#11-0081-82; RRID:AB_464915
Rat anti-mouse Granzyme B APC	eBioscience (Thermo Fisher Scientific)	CAT#17-8898-82; RRID:AB_2688068
Rat anti-mouse IL-2 APC	eBioscience (Thermo Fisher Scientific)	CAT#17-7021-82; RRID:AB_469490
Rat anti-mouse TNF-α APC	eBioscience (Thermo Fisher Scientific)	CAT#17-7321-82; RRID:AB_469508
Rat anti-mouse IFN-γ APC	BD Bioscience	CAT#554413; RRID:AB_398551
Rat anti-mouse CLEC12A PE	BioLegend	CAT#143403; RRID:AB_11126747
Rat anti-mouse CD4 FITC	Miltenyi Biotec	CAT#130-091-608; RRID:AB_244286
Rat anti-mouse CD8a APC	Miltenyi Biotec	CAT#130-091-606; RRID:AB_244291
Hamster anti-mouse CD69 PerCP-Cy5.5	eBioscience (Thermo Fisher Scientific)	CAT#45-0691-82; RRID:AB_1210703
Rat anti-mouse CD8 APC-H7	BD Bioscience	CAT#560182; RRID:AB_1645237
Rat anti-mouse CD45 PerCP	Miltenyi Biotec	CAT#130-094-962; RRID:AB_10828455
Rat anti-mouse CD62L PE	Miltenyi Biotec	CAT#130-091-794; RRID:AB_244290
Chemicals, Peptides, and Recombinant Proteins
C-type lectin receptor Fc fusion proteins	Maglinao et al., 2014, Monteiro et al., 2019, Neumann et al., 2014	N/A
pFuse-hIgG1-Fc	InvivoGen	CAT#pfuse-hg1fc2
Freestyle CHO Expression medium	Fisher Scientific	CAT#10546183
Freestyle MAX reagent	Fisher Scientific	CAT#10259172
Hemozoin	Invivogen	CAT#tlrl-hz
7-AAD viability staining solution	eBioscience (Thermo Fisher Scientific)	CAT#00-6993-50
Phorbol-12-myristate-13-acetate (PMA)	AppliChem	CAT#A0903,0001
Dihydrorhodamine (DHR)-123	Sigma-Aldrich	CAT#D1054-2MG
OVA-AlexaFluor 488	Thermo Fisher Scientific	CAT#O34781
DQ™ OVA	Thermo Fisher Scientific	CAT#D12053
LPS	Sigma-Aldrich	CAT#L4391-1MG
EndoGrade® ovalbumin	Hyglos	CAT#321001
GolgiPlug	BD Biosciences	CAT#555029
Ionomycin	Sigma-Aldrich	CAT#I0634-1MG
Critical Commercial Assays
Murine TNF-α Standard ABTS ELISA Development Kit	PeproTech	CAT#900-K54
Murine IL-12 Standard ABTS ELISA Development Kit	PeproTech	CAT#900-K97
Murine IL-2 Standard ABTS ELISA Development Kit	PeproTech	CAT#900-K108
Murine IFN-γ Standard ABTS ELISA Development Kit	PeproTech	CAT#900-K98
Granzyme B Mouse Uncoated ELISA Kit	Thermo Fisher Scientific	CAT#88-8022-88; RRID: AB_2575171
Pan T Cell Isolation Kit II mouse	Miltenyi Biotec	CAT# 130-095-130
Experimental Models: Cell Lines
mCLEC12A, hCLEC12A, mCLEC7A A5 T cell hybridoma reporter cell lines	Neumann et al., 2014	N/A
Experimental Models: Organisms/Strains
CLEC12A−/− mice	Neumann et al., 2014	N/A
OT-I C57BL/6-Tg(TcraTcrb)1100Mjb/Crl mice	Charles River	CAT#642OT1
OT-II C57BL/6-Tg(TcraTcrb)425Cbn/J mice	own breeding	N/A
Oligonucleotides
Primer for CLEC12A genotyping: WT-Fw: 5′-CTGTATGCCCTTAATACACCTCCTGC-3′	Neumann et al., 2014	N/A
KO-Fw: 5’-GGTGGGATTAGATAAATGCCTGC-3′	Neumann et al., 2014	N/A
Rv: 5’-CCATGAACAATGAGGAGAGAAGCC-3′	Neumann et al., 2014	N/A
Software and Algorithms
GraphPad Prism Version 7	GraphPad Software	N/A
FlowJo Version 10	FlowJo LLC	N/A