Figure 7.

Effect of fisetin on the LPS-induced upregulation of inflammatory mediators in the hippocampus of the adult mouse. (A) Western blot analysis of inflammatory mediators using the antibodies to TNFα, cyclooxygenase (COX)2, and interleukin-1 (IL1)-β in the mouse hippocampus. The bands were quantified using Sigma Gel software, and the differences are represented by a histogram. β-actin was used as a loading control. The density values are expressed in arbitrary units (A.U.) as the means ± SEM (number = 8 mice/group) for three repeated and reproducible independent experiments. (B) Representative images of immunofluorescent staining of TNFα in the CA3 (molecular layer and pyramidal cells) and DG (hilum and granular cells) regions of the hippocampus of the mouse brain. The results were shown as the means ± SEM (number = 5 mice/group) for three repeated and reproducible independent experiments. Magnified 10×. Scale bar = 50 μm. * shows a significant difference between control and LPS-treated groups; # shows a significant difference between LPS-treated and LPS+Fis-treated groups. Significance = p < 0.05.