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. 2019 Jun 14;8(6):850. doi: 10.3390/jcm8060850

Figure 8.

Figure 8

Effect of fisetin on the LPS-induced apoptotic neurodegeneration in adult mice brain. (A) Western blots analysis of apoptotic markers using antibodies Cyt.C, Apaf-1, caspase-9, cleaved caspase-3, and cleaved PARP-1 in the mice hippocampus. The bands were quantified using Sigma Gel software, and the differences are represented by a histogram. β-actin was used as a loading control. The density values are expressed in arbitrary units (A.U) as the means ± SEM (number = 8 mice/group) for three repeated and reproducible independent experiments. (B) Representative images of (fluoro-jade B) FJB staining in the cortex, CA1 (molecular layer and pyramidal cells), CA3 (molecular layer and pyramidal cells), and DG (hilum and granular cells) hippocampus of the mouse brain. Magnified 10×. Scale bar = 50 μm (C) Representative images of cresyl violet staining in the CA1 (molecular layer and pyramidal cells), CA3 (molecular layer and pyramidal cells), and DG (hilum and granular cells) hippocampus of the mouse brain. The presented data is relative to the control. Magnification 20×, Scale bar = 50 µm. * shows a significant difference between the control and LPS-treated groups; # shows a significant difference between LPS-treated and LPS+Fis-treated groups. Significance = p < 0.05.