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. 2019 Apr 15;5(2):32. doi: 10.3390/jof5020032

Figure 3.

Figure 3

Sexual structures of Cryptococcus gattii VGII isolates that mated with an opposite mating type strain. (a) Presence of basidia resulting from the mating of C. gattii VGIIa (MATα) strain with JEC20 (Cryptococcus neoformans serotype D, MATa); (b) formation and detachment of basidiospores from basidium resulting from the mating of C. gattii strain H0058-I-357 (MATa) with JEC21 (C. neoformans serotype D, MATα); (c) hyphae with the presence of fibule junction; and (d) formation of basidiospores from a basidium resulting from the mating of C. gattii VGIIb (MATα) strain with JEC20 (MATa). All preparations were done with calcofluor (1 mg/mL) and observed at a wavelength of 425 nm, 40×.