Table 3.
Item | Sampling Time (Weeks) | Average | SEM 1 | p-Value | |||||
---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | ||||
Gas production parameters 2 | |||||||||
PGP (mL/g dry matter (DM)) | 199 a | 201 ab | 226 b | 211 ab | 210 ab | 187 a | 206 | 5.9 | 0.001 |
c (%/h) | 7.28 | 7.17 | 6.97 | 7.04 | 7.17 | 6.88 | 7.09 | 0.181 | 0.316 |
lag (h) | 2.33 | 2.63 | 2.33 | 2.50 | 2.42 | 2.48 | 2.45 | 0.130 | 0.064 |
AGPR (mL/h) | 8.51 ab | 8.32 ab | 9.39 b | 8.81 b | 8.73 b | 7.48 a | 8.54 | 0.262 | 0.001 |
DMED (g/kg) | 324 c | 305 bc | 332 c | 313 b | 320 c | 276 a | 312 | 7.3 | <0.001 |
Fermentation parameters 3 | |||||||||
pH | 6.81 ab | 6.80 ab | 6.76 b | 6.75 b | 6.79 ab | 6.83 a | 6.79 | 0.015 | 0.004 |
Total volatile fatty acids (VFA; mmol/g DM) | 6.21 ab | 6.34 b | 6.52 b | 6.37 b | 6.41 b | 5.78 a | 6.27 | 0.25 | 0.004 |
Molar proportions (mol/100 mol) | |||||||||
Acetate | 66.8 ab | 65.9 a | 66.3 ab | 66.3 ab | 67.0 b | 66.8 ab | 66.5 | 0.23 | 0.007 |
Propionate | 23.0 ab | 23.5 ab | 23.6 b | 23.5 ab | 23.0 ab | 22.7 a | 23.2 | 0.21 | 0.014 |
Butyrate | 7.17 | 7.41 | 7.44 | 7.36 | 7.23 | 7.37 | 7.33 | 0.068 | 0.043 |
Minor VFA | 2.98 ab | 3.14 b | 2.64 a | 2.82 ab | 2.78 ab | 3.10 b | 2.91 | 0.105 | 0.011 |
Acetate/propionate (mol/mol) | 2.91 ab | 2.80 a | 2.81 ab | 2.83 ab | 2.93 ab | 2.95 b | 2.87 | 0.035 | 0.009 |
NH3 (mg/L) | 173 | 158 | 155 | 156 | 159 | 155 | 159 | 8.2 | 0.608 |
CH4 (mL/g DM) | 65.2 ab | 63.8 b | 75.0 b | 76.3 a | 65.0 ab | 61.8 a | 67.9 | 4.02 | 0.060 |
CH4/VFA (mL/mmol) | 10.1 | 9.33 | 10.3 | 10.6 | 9.91 | 10.6 | 10.1 | 0.346 | 0.112 |
ab Within each parameter, mean values with different superscripts differ (p < 0.05). 1 SEM: Standard error of the mean. 2 PGP: Asymptotic gas production (mL/g DM); c: Fractional rate of gas production (%/h); lag: Time before starting gas production; AGPR: Average gas production rate; DMED: Dry matter effective degradability calculated for a rumen particulate outflow of 4%/h. 3 Minor VFA: Calculated as the sum of isobutyrate, isovalerate and valerate.