Table 1.
Primers sequences of ARMS-PCR for Genotyping of rs2274907 A>T | ||||
---|---|---|---|---|
Fo-outer primer | F1 | 5-ACCCCTACCTTCCAGCCATCCC-3 | 403 bp | 60 °C |
Ro-outer primer | R1 | 5-CATGGGGCTGAAATGAACCCTCAGC-3 | ||
FI-T-inner primer | T allele | 5-TGCCGTCCCCCTCTGGGTAGT-3 | 251 bp | |
RIA-inner primer | A allele | 5-GTCAGCAGGGCAGCAAAGCAGA-3 | 193 bp | |
Primers sequence of Allele Specific -PCR for genotyping of rs2274908 G>A | ||||
ITLN1-F1 Wild | G allele | ACTTCCCACGCATGTCATTCTCG | 195 bp | 63 °C |
ITLN1-F2 mutant | A allele | ACTTCCCACGCATGTCATTCTCA | 195 bp | |
ITLN1-R common | Reverse | CTTTCTTGTCATGGGGCTGAAATGAAC |