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. 2019 Jun 6;9(2):30. doi: 10.3390/jpm9020030

Table 1.

Primers sequences of ARMS-PCR and Allele Specific-PCR.

Primers sequences of ARMS-PCR for Genotyping of rs2274907 A>T
Fo-outer primer F1 5-ACCCCTACCTTCCAGCCATCCC-3 403 bp 60 °C
Ro-outer primer R1 5-CATGGGGCTGAAATGAACCCTCAGC-3
FI-T-inner primer T allele 5-TGCCGTCCCCCTCTGGGTAGT-3 251 bp
RIA-inner primer A allele 5-GTCAGCAGGGCAGCAAAGCAGA-3 193 bp
Primers sequence of Allele Specific -PCR for genotyping of rs2274908 G>A
ITLN1-F1 Wild G allele ACTTCCCACGCATGTCATTCTCG 195 bp 63 °C
ITLN1-F2 mutant A allele ACTTCCCACGCATGTCATTCTCA 195 bp
ITLN1-R common Reverse CTTTCTTGTCATGGGGCTGAAATGAAC