Figure 5.

Simvastatin inhibits HCF migration after induction with HCM-derived exosomes in vitro. Exosomes were isolated from the culture medium of HCM cells pretreated with Ang II or Ang II + SIM for 24 h, and then HCF cells were incubated with human HCF cells for 24 h. HCF cell migration mediated by HCM-derived exosomes was analyzed (A), and quantified (B) by wound healing assay. Scale bar, 500 µm. * p < 0.05 vs. control from three independent experiments. To reveal simvastatin inhibition of HCF cell mobility, time-lapse confocal microscopy was used. Images were acquired (C), cell movements were measured, and displacement (μm) (D), velocity (μm/min) (E), and motility tracks (x and y axis; distance μm) (F) were quantified. * p < 0.05 vs. control from three independent experiments.