Figure 7.

DCN inhibits collagen-associated gene expression, fibroblast transformation, and cell motility in vitro. The effects of DCN on ATCA2, COL1A1, and LOXL2 expressions were examined using real-time PCR and normalized by β–actin (A–C). * p < 0.05 vs. control from three independent experiments. DCN effect on HCF cell migration was examined using time-lapse confocal microscopy. Images were acquired (D), and cell movements measured as displacement (μm) (E), velocity (μm/min) (F), and motility tracks (x and y axis; distance μm) (G), were plotted. * p < 0.05 vs. control from three independent experiments.