Figure 6.

Effect of Melanocytic Expression of RAC1^P29SIn Vivo on Tumorigenesis

(A) Schematic of the Tyr-CreER, Braf^CA and Rac1^LSL−P29S allele combination.

(B) Melanoma-free survival curves of Tyr-CreER^+/–;Braf^CA/WT;Rac1^WT/WT mice and Tyr-CreER^+/–;Braf^CA/WT;Rac1^{LSL−P29S/WT} mice were compared using log rank testing (Mantel-Cox).

(C) Effects of RAC1^P29S on tumor number in Tyr-CreER^+/–;Braf^CA/WT mice. Groups were compared using the Mann-Whitney test (n = 12–13 mice per genotype).

(D) Schematic of the Tyr-CreER, Pten^F, Braf^CA, and Rac1^LSL-P29S allele combination.

(E) Overall survival curves of Tyr-CreER^+/–;Pten^F/WT;Braf^CA/WT;Rac1^WT/WT mice and Tyr-CreER^+/–;Pten^F/WT;Braf^CA/WT;Rac1^{LSL−P29S/WT} mice were compared using the log rank testing (Mantel-Cox).

(F) Effects of RAC1^P29S on tumor number in Tyr-CreER^+/–;Pten^F/WT;Braf^CA/WT mice. Groups compared using Mann-Whitney testing (n = 5–9 mice per genotype).

(G) Schematic of the Tyr-CreER, Trp53^F, Braf^CA, and Rac1^LSL−P29S allele combination.

(H) Melanoma-free survival curves of Tyr-CreER^+/–;Trp53^F/F;Braf^CA/WT;Rac1^WT/WT mice and Tyr-CreER^+/–;Trp53^F/F;Braf^CA/WT;Rac1^{LSL−P29S/WT} mice were compared using log rank testing (Mantel-Cox).

(I) Effects of RAC1^P29S on tumor number in Tyr-CreER^+/–;Trp53^F/F;Braf^CA/WT mice. Groups compared using an unpaired two-tailed t test (n = 7–9 mice per genotype).

(J) Representative photographs and histology of the dorsal skin of various mouse melanoma models. All mice are Tyr-CreER^+/–, with additional alleles indicated. Time since 4OHT treatment is indicated.

For all graphs: bars represent means ± SD. See also Figure S6.