Fig. 1.

FcRn interacts with HCMV US11. a, b The cell lysates from HeLa^FcRn+US11 (lane 1), HeLa^FcRn (lane 2), HeLa^US11 (lane 3), and HeLa control cells (lane 4) were immunoprecipitated by mAb anti-HA for US11 or anti-FLAG for FcRn. The immunoprecipitates were subjected to western blotting with anti-FLAG or HA mAb as indicated. Cell lysate from each sample with equal amounts of total protein (input, 20 μg) were blotted with the indicated Abs. c Co-localization of FcRn and US11 in HeLa^FcRn+US11 cells. HeLa^FcRn cells or HeLa^US11 cells were used as a control. Puncta that appear yellow in the merged images (right panel) indicate co-localization of FcRn with US11 protein. The nuclei were stained with DAPI (blue). Scale bar: 10 μm. d, e Cell lysates from HeLa^HFE+US11 (lane 1), HeLa^HFE (lane 2), HeLa^US11 (lane 3), and HeLa control cells (lane 4) were immunoprecipitated with mAb anti-HA for US11 or anti-FLAG for HFE, respectively. The immunoprecipitates were subjected to western blotting with anti-FLAG or HA mAb as indicated. The cell lysates (input) were blotted as control. f, g US11 interacts with FcRn in HCMV-infected human primary umbilical vein endothelial cells (HUVEC). HUVEC were infected with HCMV at an MOI of 5. At day 2 p.i., the cell lysates from infected or mock-infected HUVEC were immunoprecipitated with anti-US11 Ab (f) or anti-FcRn Ab (g). The immunoprecipitates were subjected to 12% SDS-PAGE electrophoresis under reducing conditions, then transferred to a nitrocellulose membrane for western blotting with anti-FcRn or US11 Ab as indicated. The cell lysates (20 μg) were blotted as controls