Table 1.
Experimental design for dose (L-Arg) and embronic stage (eighth day, 14th day, and 18th day) fixation.
| Group | Dosage | No. of Eggs & No. of Replication | Total No. of Eggs |
|---|---|---|---|
| 1C | Control | 20 eggs × 4 replicates | 80 |
| 1C1 (8th day) | PBS/100 µL/egg | 20 eggs × 4 replicates | 80 |
| 1T1 (8th day) | 100 µg/100 µL/egg | 20 eggs × 4 replicates | 80 |
| 1T2 (8th day) | 1000 µg/100 µL/egg | 20 eggs × 4 replicates | 80 |
| 1T3 (8th day) | 2500 µg/100 µL/egg | 20 eggs × 4 replicates | 80 |
| 2C1 (14th day) | PBS/100 µL/egg | 20 eggs × 4 replicates | 80 |
| 2T1 (14th day) | 100 µg/100 µL/egg | 20 eggs × 4 replicates | 80 |
| 2T2 (14th day) | 1000 µg/100 µL/egg | 20 eggs × 4 replicates | 80 |
| 2T3 (14th day) | 2500 µg/100 µL/egg | 20 eggs × 4 replicates | 80 |
| 3C1 (18th day) | PBS/100 µL/egg | 20 eggs × 4 replicates | 80 |
| 3T1 (18th day) | 100 µg/100 µL/egg | 20 eggs × 4 replicates | 80 |
| 3T2 (18th day) | 1000 µg/100 µL/egg | 20 eggs × 4 replicates | 80 |
| 3T2 (18th day) | 2500 µg/100 µL/egg | 20 eggs × 4 replicates | 80 |
Note: In Ovo Injection and Treatment Groups.