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. 2019 Jul 10;12:65. doi: 10.1186/s13041-019-0473-0

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Phosphorylation of FMRP is the switch for NMDAR mediated translation. a Immunoblots for phospho FMRP (at S499) and total FMRP from synaptoneurosomes after NMDAR stimulation. Quantification of the ratio of phospho-FMRP to total-FMRP normalized to tuj1 for NMDAR stimulation (n = 6, paired Student’s t test, ±SEM).b Immunoblots for FLAG showing overexpression of FMRP-S499D, FMRP-S499A and tubulin from Neuro 2a cells. Bottom panel: Immunoblots for FLAG-FMRP and AGO2 after AGO2 immunoprecipitation from Neuro 2a cells transfected with FMRP-S499D or FMRP-S499A. Quantitative analysis of AGO2 association with FLAG-FMRP following AGO2 immunoprecipitation normalised to levels in the FMRP-S499D overexpressing cells. (n = 3, unpaired Student’s t-test, ±SEM). c Immunoblots for FMRP showing overexpression of FMRP-S499D, FMRP-S499A and tubulin from Neuro 2a cells. Bottom panel: Immunoblots for AGO2 and MOV10 after MOV10 immunoprecipitation from Neuro 2a cells transfected with FMRP-S499D or FMRP-S499A. Quantitative analysis of AGO2 association with MOV10 following MOV10 immunoprecipitation normalised to levels in the FMRP-S499D overexpressing cells. (n = 3, unpaired Student’s t-test, ±SEM). d Distribution of MOV10 separated on a linear sucrose gradient from Neuro 2a cells untransfected (UT) or transfected with FMRP-S499D based on immunoblots shown below followed by quantification of MOV10 in polysomes (n = 3, unpaired Student’s t-test, ±SEM). e Model illustrating the role of MOV10 in response to NMDAR stimulation which is mediated by FMRP and its phosphorylation status