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. 2019 Jul 10;38:298. doi: 10.1186/s13046-019-1287-8

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Knockdown of ATG4C suppressed autophagy and autophagic flux. a, b ATG4C stable knockdown glioma cells were established by lentivirus infection, and the interference efficiency was detected by Western blot analysis. c Protein expression of LC3-II and P62 in glioma cells transfected with si-ATG4C. d, e Knockdown of ATG4C on the expression of LC3-II in the presence of BafA1. f Transmission electron microscopy observation of cellular ultrastructure tin T98G cells by ATG4C depletion. Arrows indicate autophagic vacuoles. *p < 0.05, **p < 0.01, ***p < 0.001, NS: not significant