Figure 2.

Isolation of the potential promoter and putative signal peptide of the HASP1 gene and selection of transformants by PCR analysis with genomic DNA. (a) Schematic representation of the HASP1 gene location on chromosome 14. The grey arrows indicate the potential promoter of the HASP1 gene. Black arrows indicate the gene orientation. (b) Nucleotide sequence of the potential promoter region of the HASP1 gene. (c) PCR amplification of transgenes from the genomic DNA of transformants. The numbers indicate independent transgenic lines for each construct. Full-length agarose gels are presented in Supplementary Fig. S4. Asterisk shows the nonspecific PCR products. M, molecular size marker.