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. 2019 Jul 9;9:9941. doi: 10.1038/s41598-019-45786-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Growth curves of transgenic P. tricornutum and levels of GFP transcript in the selected transgenic lines. (a) Cell growth curves of P. tricornutum cultures. The selected transgenic lines were grown for 22 days. (b) Relative levels of GFP mRNA in the selected transgenic lines. GFP expression levels were normalised to TBP (TATA-box binding protein) expression. Data are expressed as the mean ± SD of three replicates. The asterisk (*) and hash (#) indicate statistically significant differences vs. the transformant groups of the negative (promoter-less GFP) and positive controls, respectively. ns, not significant (p > 0.05); *^/#p < 0.05, **^/##p < 0.01, ***^/###p < 0.001 (ANOVA).