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. 2019 Jul 9;19:127. doi: 10.1186/s12871-019-0788-5

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — TNF-α could decrease the expression of occludin in hCMEC/D3 cells. a The hCMEC/D3 cells were treated with different concentrations of TNF-α (1, 10, 25, 50 and 100 ng/ml) for 8 h. Left side shows the image of a representative Western blot for occludin; right side is the plot of normalized ratios of optical densities. b The hCMEC/D3 cells were incubated with TNF-α (10 ng/ml) for different hours (2, 4, 8, 12, 24 h). Left side is the image of a representative Western blot for occludin; right side is the plot of normalized ratios of optical densities. c The cell viability of hCMEC/D3 cells after TNF-α treatment. β-actin was served as internal loading control. Data were represented as the mean ± SD. *p < 0.05, compared with the control group. Each assay was performed in five replicates