Fig. 6.

IPO5-mediated nuclear import of RASAL2 is required for RAS pathway activation and CRC development a Western blotting was used to determine the expression of ERK, p-ERK, AKT, and p-AKT upon knockdown or overexpressing of IPO5 in CRC cells. b The NLS mutant of RASAL2 resulted in reversing the IPO5-mediated RAS signaling activation showed by Western blotting. c and d The recovery effect of NLS mutant on the IPO5-mediated CRC cells migration and proliferation ability were detected by colony formation and transwell respectively. Scale bars, 200 μm. e Subcutaneous tumorigenesis in nude mice was applied to explore the impact of NLS mutant on tumorigenicity of IPO5 in vivo. Data represent the mean ± SD of 3 independent experiments. *P < 0.05. f Tumor xenograft tissues were embedded in paraffin and then assessed for the expression of Ki-67 and RASAL2. Scale bars, 200 μm. g Correlation of nuclear RASAL2 expression and IPO5 expression in colorectal cancer tissue was evaluated by Immunohistochemical. Scale bars, 50 μm