Figure 2.

The impact of fMLF on platelet adhesion and aggregation. A, different concentrations of fMLF were used to analyze their influence on human platelet adhesion on immobilized collagen under static conditions. Data represent mean ± SEM (n = 10). B, the effect of fMLF on the modulation of thrombus formation was analyzed by using human DiOC6‐labeled whole blood that was preincubated with nonformylated MLF or fMLF (5 μmol/L) for 10 min prior to perfusion over collagen‐coated (400 μg/mL) Vena8™ Biochips. Images shown are representative of three separate experiments (10× magnification; scale bar ‐ 10 μm). Likewise, the effects of fMLF on C, cross‐linked collagen‐related peptide (CRP‐XL)‐induced, , D, collagen‐induced, or E, thrombin‐induced platelet activation were measured using isolated human platelets by optical aggregometry. Data represent mean ± SEM (n = 5). The statistical significance was calculated by 1‐way ANOVA followed by Bonferroni's correction in most of the experiments except the data shown in panels B, which were analyzed by 2‐tailed unpaired Student t test (*P <0.01, **P <0.001, and ***P <0.0001). fMLF, N‐formyl‐methionyl‐leucyl‐phenylalanine; MLF, methionyl‐leucyl‐phenylalanine