Figure 3.

(a) Treatment of LNCaP cells with ADT + THD results in apoptosis. LNCaP cells were incubated bicalutamide (10 μM), THD (10 μM), or bicalutamide + THD for 24 h for cell cycle analysis of PI‐stained cells. (b)–(c) LNCaP‐Western Blots of cell cycle and apoptotic indicators. Cells from the four treatment groups as in A were analyzed by WB for several indicators of DNA damage/apoptosis and cell cycle arrest/proliferation. (d)–(e) TRAMP‐C2‐Western blots of several proteins involved in cell cycle and apoptosis. [Color figure can be viewed at wileyonlinelibrary.com]