Figure 4.

(a)–(e) Combination bicalutamide and THD suppresses growth of LNCaP xenografts. A. Time course of tumor growth of LNCaP cells xenografts in 4 treatment groups. Treatment started after 2 weeks of implantation when the tumors measure ~200 mm³. Bicalutamide was injected IP twice/week and THD IP an alternate days. (b) Examples of tumor size at the end of the experiment. (c) Tumor weights at the end of the experiment. (d) Same as in (a) but the mice were treated with THD given orally an alternate days. Same as in C but the mice were treated with THD given orally. (e) Tumor weights. (f) Proteins extracted from the tumors of two representative mice in each group were analyzed by western blot for the indicated key proteins of cell cycle checkpoint and also for pAKT and histone H3. (g) Representative sections from tumors resected from mice in the 4 treatment groups analyzed by IHC for pNek1. Note the absence of pNek1 stain in the bicalutamide+THD tumor, consistent with the results shown in F. [Color figure can be viewed at wileyonlinelibrary.com]