Figure 6.

(a) Overexpression of wt‐Nek1, but not the T141A mutant, counters the growth arrest from bicalutamide and the apoptosis induced by combination with THD. LNCaP parental cells and overexpressing Nek1 (wt or T141A mutant) were plated at low density and incubated for 1 week without or with bicalutamide and +/− THD. (b) LNCaP overexpressing Nek1‐T141A form slow‐growing tumor xenografts which remain sensitive to ADT. Time course of tumor growth LNCaP cells xenografts in mice treated or not with bicalutamide. Treatment started after 2 weeks of implantation when the tumors measure ~100 mm³ (note that at this time, tumors from parental LNCaP had already reached 200 mm³—Fig. 4). (c) Examples of tumor size at the end of the experiment. (d) Tumor weights at the end of the experiment. (e) Body weights of mice treated or not with bicalutamide. [Color figure can be viewed at wileyonlinelibrary.com]