Figure 1.

SNX siRNA screening to identify novel angiogenic factors in HUVECs. (a) Confocal images of tube formation. HUVECs seeded on collagen I gel were treated with the control or SNXs siRNA (SNX1‐33) and packed on collagen I followed by VEGF stimulation for 66 hr. HUVECs were visualized by the staining of F‐actin with rhodamine‐labeled phalloidin. Bars, 200 µm. (b) The quantitation of (a). A total of 10 tubes were analyzed. Data shows are the mean ± SEM. The blue and red bars indicate the tube length of sicontrol‐ and siSNX9‐treated HUVECs, respectively. HUVEC: human umbilical vein endothelial cells; SEM: standard error of mean; siRNA: small interfering RNA; SNX: sorting nexin; VEGF: vascular endothelial growth factor [Color figure can be viewed at wileyonlinelibrary.com]