Figure 4.

Energy expenditure upon chemogenetic activation of LH LepR neurons. (A) CNO (1.0 mg/kg) injections in LepR‐cre mice (n = 8) increased locomotor activity. (B) Examples of thermal images from a thermosensitive camera in which CNO‐injected LH VGAT mouse is standing on hind legs with eyes facing upward. (C) CNO increased eye temperature compared with saline injections. (D) Area under the curve of panel C. Mean ± SEM. Individual points are plotted as grey squares. *P < 0.05, **P < 0.01. [Colour figure can be viewed at wileyonlinelibrary.com]