Table 2.
Selected genes for transcript profiling based on biological function and expression profiles of corresponding proteins
| Gene | Biological function | Spot no. /cluster | Primer sequence (5′ – 3′) | PCR product size (bp) | GeneBank accession no. |
|---|---|---|---|---|---|
| Actin | Reference gene | – |
F:TGTTCCCCGGAATTGCTGATAGAATGAGC R:TTGGAAAGTGCTGAGAGATGCCAAAATGGAG |
146 | AJ012685 |
| Carbonic anhydrase | Photosynthesis | 45 / 4 |
F: TTGAAGTGAAGGAAACC R: AGAAGAAATGGGAAAGG |
195 | LOC101498889 |
| Glycerate dehydrogenase | Amino acid & nitrogen metabolism | 39 / 3 |
F: GAAAGACTCGCCAAGA R: GGGCTCATCCTCAAAC |
141 | LOC101496862 |
| Heat shock 70 kDa protein | Stress responsive | 4 / 3 |
F: CTTGATGTAACGCCACTT R: GTCCACCAGATGACCTAATA |
346 | LOC101502051 |
| L-ascorbate peroxidase | Stress responsive | 55 / 5 |
F: AAATCTTACCCAACCGTCA R: CAACAACACCACCCAACT |
304 | LOC101497640 |
| ATP-dependent zinc metalloprotease FTSH2 | Protein synthesis and degradation | 2 / 4 |
F: AAAGAACAACCGTGAAGCAA R: CGGCGACTGGTAATGGA |
152 | LOC101512859 |
| 6-phosphogluconate dehydrogenase | Other metabolisms: PP | 15 / 4 |
F: TGACAGCAAGGCAAACAACTC R: CAGGCATACGACAGAAACCC |
112 | LOC101509898 |