Fig. 1.

FACS data of CD4⁺CD25⁻ or CD4⁺CD25^–FoxP3⁻ T cells from spleen of BALB/c and FoxP3eGFP mice. Cells were treated in vitro for 72 h with CD3/CD28 antibodies as described in the “Methods” section. Plots show CD25-PE on the y-axis and FoxP3-APC or FoxP3eGFP on the x-axis. Quadrant statistics noted are percent of live CD4⁺ gate. a–d Mean values of % CD4⁺CD25⁺Foxp3⁺ from 5 independent experiments show SM16 effects on TGF-β-induced Treg conversion (e). Significance between control and experimental groups were determined by Student’s t-test (*, P < 0.05)