Table 1.

The absolute cell number of CD4⁺ T cell subsets calculated based on flow cytometry analysis of lymph nodes and spleen of RagKO mice reconstituted with CD4⁺GFP⁻T cells and maintained on control or SM16 diet for 21 days

	Absolute cell number per mouse (105)
	Lymph node		Spleen
Treatment	Control (%)	SM16 (%)	Control (%)	SM16 (%)
Total cell recovery
All CD4+	1.18 ± 0.5	13.5 ± 1.4	21.7 ± 0.05	88.8 ± 1.90
CD4+ CD25+ Foxp3+ (Treg)	0.1 ± 0.06 (1.4)	0.4 ± 0.03* (3.6)	0.4 ± 0.2 (3.7)	2.5 ± 1.03a (3.8)
CD4+ CD25+ Foxp3− (activated T cell)	0.08 ± 0.04 (8.3)	0.7 ± 0.04# (6.4)	1.6 ± 1.3 (3.9)	2.5 ± 0.7b (2.7)
CD4+ CD25− Foxp3− (other)	1.0 ± 0.5 (91.3)	12.4 ± 6.3& (90.0)	19.7 ± 0.01 (94.0)	83.8 ± 0.01c (93.5)

Absolute numbers (AN) are presented as the mean trait value ± SEM. The % was determined based on average of ANs from 4 independent experiments

*p value ≤ 0.05, as compared to RagkO on control diet

^#p value ≤ 0.05, as compared to RagkO on control diet

^&p value ≤ 0.05, as compared to RagkO on control diet

^ap value ≤ 0.05, as compared to RagkO on control diet

^bp value ≤ 0.05, as compared to RagkO on control diet

^cp value ≤ 0.05, as compared to RagkO on control diet