Figure 1.

Effects of CD4⁺ and CD8⁺ T lymphocyte depletion on survival and morbidity in animals immunized with pE1D2 or pcTPANS1 and challenged with DENV2. Schematic timeline representation of the experiment (A). BALB/c mice intramuscularly immunized with pE1D2 or pcTPANS1 (n = 10) were intraperitoneally inoculated with anti-CD4 or anti-CD8 antibodies at days 4 and 2 prior to intracerebral virus challenge (40 x LD₅₀ of a neuroadapted DENV2). The control group (DENV) consisted of non-immunized mice (n = 8) inoculated with DENV2. All experimental groups were monitored for 21 days after challenge to record the survival rates (B) and degree of morbidity (C). Asterisks indicate significant differences using Log-Rank (Mantel-Cox) test in (B) or one-way ANOVA test in (C) (*p < 0.05; **p < 0.001; ***p < 0.0001). Lines in (C) represent the median of each group.