Figure 3.

Differential IGHV usage between isotypes. (A) Heat map indicating the relative frequency of all IGHV genes among unique VDJ sequences per isotype. For each isotype, the frequencies shown add up to 100%. (B) Pairwise comparison of cumulative IGHV gene distance between two isotypes. For each IGHV gene, the fractions of VDJ sequences containing that IGHV gene within an isotype were calculated, and the numerical difference of these fractions between two isotypes was determined per IGHV gene. For each pairwise comparison between isotypes, all differences of IGHV gene fractions were added to obtain their cumulative distance.